Journal of Chromatography & Separation Techniques

Journal of Chromatography & Separation Techniques
Open Access

ISSN: 2157-7064

Yong Wang

Yong Wang

China

Publications
  • Research Article
    Chromatographic and Molecular Simulation Study on the Chiral Recognition of Atracurium Besylate Positional Isomers on Cellulose Tri-3, 5 Dimethylphenycarbamate (CDMPC) Column and its Recognition Mechanism
    Author(s): Theophilus Ndorbor, Yong Wang, Ding Huaijing, Ding Zhizhang, Jacob A Kolawole and He Hua Theophilus Ndorbor, Yong Wang, Ding Huaijing, Ding Zhizhang, Jacob A Kolawole and He Hua

    A baseline separation was achieved for the direct HPLC separation of atracurium besylate stereoisomers; atracurium trans-trans, atracurium trans-cis, and atracurium cis-cis, on a Cellulose tri- 3, 5-dimethylphenycarbamate (CDMPC) column. Acetonotrile (ANC) and Potassium hexaflourophosphate (KPF6) were used as mobile phase. The effect of organic modifier, pH, buffer concentration, temperature, and flow rate on retention time and enantioselectivity, was investigated. Binding energy differences, mode of interaction as determined by computer simulation method, were used to elucidate chiral recognition mechanism and explain the effect of organic modifier on enantioselectivity. The result suggests that the isomers of atracurium besylate could be well resolved on a CDMPC column by a 50:50 ANC: KPF6 (0.1 M, pH 3.0-3.5) mobile phase in the temperature range of 30-38°C, at a flow rate betwe.. View More»
    DOI: 10.4172/2157-7064.1000176

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