Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
+44 1223 790975
ISSN: 0974-276X
+44 1223 790975
Yogeshwer Shukla
Proteomics Laboratory, Indian Institute of Toxicology Research (CSIR),
Mahatma Gandhi Marg, Lucknow 226001 (U.P.)
India
Research Article
Plasma Protein Profiling of Breast Cancer Patients of North Indian
Population: A Potential Approach to Early Detection
Author(s): Deepika Arora, Zafar Mahmood, Jasmine George, Alok Kumar Yadav, Shailendra Kumar, Uma Shanker Singh and Yogeshwer ShuklaDeepika Arora, Zafar Mahmood, Jasmine George, Alok Kumar Yadav, Shailendra Kumar, Uma Shanker Singh and Yogeshwer Shukla
Study background: Over the last two decades worldwide the mortality due to breast cancer (BC) raises catastrophically. Unfortunately, clinicians are still seeking for reliable resources for early speculations. Recently, the approach of investigative plasma based molecular markers builds a new hope for the early stage diseases.
Methods: Blood samples of healthy and BC individuals from North Indian women (n=25) were collected. In order to increase the detecting sensitivity of protein of interest, the pooled plasma samples were subjected to Multiple affinity removal system (MARS) Hu-14 and PROTEOPREP 20 and further processed by 2D-PAGE/LCMS/ MS analysis. Finally, the levels of selected proteins were independently estimated by western blotting and immunohistochemistry.
Results: The comparative 1D-SDS page and 2DE gel a.. View More»
DOI:
10.4172/jpb.1000267