Journal of Proteomics & Bioinformatics

Journal of Proteomics & Bioinformatics
Open Access

ISSN: 0974-276X

+44 1223 790975

Yasmin Ahmad

Yasmin Ahmad
Defence Research & Development Organization (DRDO),
Ministry of Defence, Timarpur, Lucknow Road, Delhi, 110054

  • Research Article
    An Effective Method for the Analysis of Human Plasma Proteome using Two-dimensional Gel Electrophoresis
    Author(s): Yasmin Ahmad and Narendra SharmaYasmin Ahmad and Narendra Sharma

    Proteome analysis of plasma is increasingly leading to biomarker discovery of human diseases. However the highabundant proteins, excess of salt and lipid in plasma makes the analysis very challenging. Therefore it is necessary to improve the sample preparation procedures before/after the two-dimensional gel electrophoresis analysis of plasma proteins. The objective of this study was to develop a reproducible method by examining the following parameters: (1) depletion of the high-abundant proteins (2) effect of different precipitation methods (3) comparing optimised rehydration buffer using modified Taguchi method with the standard rehydration buffer and (4) comparing the effects of different staining methods. Our results showed that the depletion of two high-abundant proteins improved the visualization of less abundant proteins present in human plasma and precipitatio.. View More»
    DOI: 10.4172/jpb.1000111

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