Journal of Leukemia
Open Access
ISSN: 2329-6917
ISSN: 2329-6917
Yan Zhao
Japan
Research Article
Effects of Resveratrol and Vitamin C on the Self-Renewal Capacity of Blast Progenitors in Hematologic Malignancies
Author(s): Yi Zhang, Yan Zhao, Kazuma Miyahara, MaiMai Shimada, Ken-ichi Tanaka, Hiroyuki Hayashi, Noriko Ihara and Ikuo MurohashiYi Zhang, Yan Zhao, Kazuma Miyahara, MaiMai Shimada, Ken-ichi Tanaka, Hiroyuki Hayashi, Noriko Ihara and Ikuo Murohashi
Although a large number of phytochemicals has been shown to inhibit the growth of leukemic and cancer cells, their effects on the self-renewal (SR) capacity of the malignant stem/progenitor cells were not examined. In seven hematologic malignant cell lines including U-937, Mo7e, K-562, HL-60, U-266, Raji and Daudi, the SR capacity of blast progenitors was assessed by measuring cumulative clonogenic cells in suspension, whereas the terminal division of blast progenitors was assessed by blast colony formation (BCF) in methylcellulose. Resveratrol and vitamin C (Vit-C) with mean IC50 values of 41 and 514 ?M for inhibiting BCF were added twice per week to cells in long-term suspension at 10 and 300 ?M, respectively. Vit-C induced slight increase in senescence or necrosis rates, marked G2/M arrest and significant inhibition of telomerase activity in two, one and five cell lines, respectively... View More»
DOI:
10.4172/2329-6917.S1-006
