Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
+44 1223 790975
ISSN: 0974-276X
+44 1223 790975
Xolani Henry Makhoba
South Africa
Mini Review
Molecular Chaperone Assisted Expression Systems: Obtaining Pure
Soluble and Active Recombinant Proteins for Structural and Therapeutic
Purposes
Author(s): Xolani Henry Makhoba, Ofentse Jacob Pooe and Mathews Simon MthembuXolani Henry Makhoba, Ofentse Jacob Pooe and Mathews Simon Mthembu
For many years recombinant protein production has been at the center of biosciences used for structural and therapeutic purposes. The production of recombinant proteins in foreign host system such as E. coli has been a biggest challenge. This has brought negative impact on the ongoing search for alternative drugs and vaccines for diseases such as schistosomiasis, African sleeping sickness and malaria. At present, no system is available to produce various recombinant proteins from different sources. Hence, the search for a universal system that can be utilized for any recombinant protein is crucial. The basic requirements for high quality protein production in a pure active and soluble form have led to the use of molecular chaperones as supplement during recombinant proteins production in E. coli. Molecular chaperones are proteins that are known to assist the newly synthesized proteins.. View More»
DOI:
10.4172/jpb.1000371