Journal of Proteomics & Bioinformatics
Open Access

Wouter Ouwerkerk

Publications

• Research Article
  Alternative Splice Variants in Gene Expression Values in Patients with Marfan's Syndrome
  Author(s): Wouter Ouwerkerk and Aeilko H ZwindermanWouter Ouwerkerk and Aeilko H Zwinderman
  
  Background: Alternative splicing of messenger RNAs provides cells with the opportunity to create protein isoforms of a multitude of functions from a single gene by excluding or including exons during post-transcriptional processing. Reconstructing the contribution of these splice variants on the total amount of gene expression remains difficult. Methods: We introduced a probabilistic formulation of the alternative splicing reconstruction problem using a finite mixture model, and provide a solution based on the maximum likelihood principle. Our model is based on the assumption that the expected expression level of exons in a particular splice variant is the same for all exons in that variant but allows for measurement error. In this algorithm the expression in a patient can be written as a weighted sum of the number of splice variant mixture multivariate Gaussian densities. W.. View More»
  DOI: 10.4172/jpb.1000346

  Abstract PDF

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