Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
ISSN: 0974-276X
Weidong Zhou
Tanzania
Research Article
Discovery of Infectious Disease Biomarkers in Murine Anthrax Model Using Mass Spectrometry of the Low-Molecular-Mass Serum Proteome
Author(s): Aarthi Narayanan, Weidong Zhou, Mark Ross, Jane Tang, Lance Liotta, Emanuel Petricoin, Fatah Kashanchi, Charles Bailey and Serguei PopovAarthi Narayanan, Weidong Zhou, Mark Ross, Jane Tang, Lance Liotta, Emanuel Petricoin, Fatah Kashanchi, Charles Bailey and Serguei Popov
A novel mass spectrometry (MS)-based approach to the identification of host-derived biomarkers (BMs) in the circulating low-molecular-mass (LMM) fraction ( <25 kDa) of blood proteome was tested in a murine model . DBA2/J mice were challenged intraperitonially with spores of either the toxigenic B. anthracis Sterne strain (pXO1 + , pXO2 - ) that is virulent in DBA/2 mice or the nontoxigenic, non-virulent delta Sterne strain (pXO 1 - , pXO2 - ). Serum samples were obtained at multiple time points and seperated by continuous flow denaturing gel electrophoresis followed by Coomassie staining to i so- late the LMM archive for subsequent MS identificati on. Peptide fragments derived from more than 200 protei ns displayed low-variance differential abundances betw een lethal and non-lethal challenges. Several proteins from the.. View More»
DOI:
10.4172/jpb.1000101