Journal of Chromatography & Separation Techniques
Open Access
ISSN: 2157-7064
+44 1300 500008
ISSN: 2157-7064
+44 1300 500008
Vijay Kumar
India
Research Article
Anti-Human IgG-Horseradish Peroxidase Conjugate Preparation and its Use in ELISA and Western Blotting Experiments
Author(s): Ramesh Kumar K, Sneha Xiavour, Swarna Latha, Vijay Kumar and SukumaranRamesh Kumar K, Sneha Xiavour, Swarna Latha, Vijay Kumar and Sukumaran
Studies of our experiment are related to the binding of enzymes to antibodies. This involves the formation of a stable, covalent linkage between an enzyme & antibody. We used different Enzymes for this studies which are altered in binding action. Enzymes we used-e.g. Horseradish peroxidase (HRPO), urease, or alkaline phosphatase. Studies of Enzyme and an antigen-specific monoclonal or polyclonal antibody in which neither the antigen-combining site nor the active site of the enzyme is functionally altered. The enzyme most commonly used in the immunoreagent (the antibody enzyme conjugate) preparation is horseradish peroxides. This enzyme is cheap and can be attached to the immunoreagent by a variety of methods. Moreover many chromogenic substrates for it are also available. Our Experiment studies on conjugation of Horseradish Peroxidase (HRPO) to antibody (anti human IgG) will be ca.. View More»
DOI:
10.4172/2157-7064.1000211