Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
+44 1223 790975
ISSN: 0974-276X
+44 1223 790975
Vijay K Singh
Armed Forces Radiobiology Research Institute,
8901 Wisconsin Ave Bethesda, MD 20889-5603
Tanzania
Research Article
Personalized Radioproteomics: Identification of a Protein Biomarker
Signature for Preemptive Rescue by Tocopherol Succinate in CD34+
Irradiated Progenitor Cells Isolated from a Healthy Control Donor
Author(s): Anjali Srivastava, Ximena Leighton, Ofer Eidelman, Joshua Starr, Catherine Jozwik, Meera Srivastava, Harvey B Pollard and Vijay K SinghAnjali Srivastava, Ximena Leighton, Ofer Eidelman, Joshua Starr, Catherine Jozwik, Meera Srivastava, Harvey B Pollard and Vijay K Singh
Tocopherol succinate (TS) has been shown to protect mice against acute radiation syndrome, however, its exact mechanism of action and its possible use in humans has not yet been evaluated. Our approach has been to test the radioprotectant properties of TS on CD34-positive stem cells from healthy volunteers. We hypothesize that a radioproteomics strategy can identify a drug-dependent, personalized proteomics signature for radioprotection. To directly test the radioproteomics hypothesis, we treated human CD34-positive stem cells with 20 μM TS for 24 h, and then exposed the cells to 2 Gy of cobalt-60 gamma-radiation. We isolated protein from all cultures and used a high throughput Antibody Microarray (AbMA) platform to measure concentrations of 725 low abundance proteins. As an in vivo control, we also tested mouse CD34-positive stem cells using the same preemptive TS paradigm on pr.. View More»
DOI:
10.4172/jpb.1000349