Journal of Chromatography & Separation Techniques
Open Access
ISSN: 2157-7064
+44 1300 500008
ISSN: 2157-7064
+44 1300 500008
Vasconcelos André Belico de
University of Uberaba,
Av. Nenê Sabino 1800, Uberaba, Minas Gerais, CEP 38061-500
Brazil
Research Article
Gel Filtration Chromatography Technique as Tool of Simple Study Seminal Plasma Proteins in Domestic Animals
Author(s): Vasconcelos André Belico de, Oliveira Jamil Silvano de and Lagares Monique de AlbuquerqueVasconcelos André Belico de, Oliveira Jamil Silvano de and Lagares Monique de Albuquerque
Seminal fluid is the liquid component of sperm, providing a safe surrounding for spermatozoa. The seminal plasma has the feature common to many other body fluid, characterized by a high dynamic range of proteins, which visualizes physiological and biochemical processes of semen. As in other body fluids, it is convenient to distinguish in the seminal plasma between proteins and non-proteins. The molecular exclusion chromatographic technique presents important points to constitutional proteins preservation, in addition to the conventional phenomenon; witch exclusion hydrophobic interactions can provide a higher resolution in the chromatogram and do not provide nonspecific interactions between protein structures. The aim was the chromatography profile to simple study of proteins seminal plasma domestic animals (Stallions, Canine, and Goat), by the technique of gel filtration chromatograp.. View More»
DOI:
10.4172/2157-7064.1000281