Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
+44 1223 790975
ISSN: 0974-276X
+44 1223 790975
Udensi K Udensi
RCMI Center for Environmental Health, College of Science, Engineering and Technology,
Jackson Mississippi 39217
Tanzania
Research Article
Proteomics-Based Identification of Differentially Abundant Proteins from
Human Keratinocytes Exposed to Arsenic Trioxide
Author(s): Udensi K Udensi, Alan J Tackett, Stephanie Byrum, Nathan L Avaritt, Deepanwita Sengupta, Linley W Moreland, Paul B Tchounwou and Raphael D IsokpehiUdensi K Udensi, Alan J Tackett, Stephanie Byrum, Nathan L Avaritt, Deepanwita Sengupta, Linley W Moreland, Paul B Tchounwou and Raphael D Isokpehi
Introduction: Arsenic is a widely distributed environmental toxicant that can cause multi-tissue pathologies. Proteomic assays allow for the identification of biological processes modulated by arsenic in diverse tissue types.
Method: The altered abundance of proteins from HaCaT human keratinocyte cell line exposed to arsenic was quantified using a label-free LC-MS/MS mass spectrometry workflow. Selected proteomics results were validated using western blot and RT-PCR. A functional annotation analytics strategy that included visual analytical integration of heterogeneous data sets was developed to elucidate functional categories. The annotations integrated were mainly tissue localization, biological process and gene family.
Result: The abundance of 173 proteins was altered in keratinocytes exposed to arsenic; in which 96 proteins had increased abundance while 77 proteins.. View More»
DOI:
10.4172/jpb.1000317