Journal of Proteomics & Bioinformatics

Journal of Proteomics & Bioinformatics
Open Access

ISSN: 0974-276X

+44 1223 790975

Tek Chand Bhalla

Tek Chand Bhalla

Summer Hill, Shimla-171005, Himachal Pradesh

  • Research Article
    Microbial Carboxylesterases: An Insight into Thermal Adaptation Using In Silico Approach
    Author(s): Samta Sood, Nikhil Sharma and Tek Chand BhallaSamta Sood, Nikhil Sharma and Tek Chand Bhalla

    Carboxylesterases (CE’s), are a group of esterases that catalyze the hydrolysis of carboxylic ester molecule to form alcohol and carboxylic acid in the presence of water. In silico analysis of ten carboxylesterases (E.C. sequences from mesophilic and thermophilic organisms for various physiochemical parameters and amino acid comparison has been done. Ten sequences, five from each group were retrieved from NCBI and were aligned using multiple sequence alignment tool MUSCLE. The phylogenetic relationship between the two groups has been found using maximum parsimony method of MEGA-6. These sequences were further analyzed using online ProtParam ExPASy tool for some important physicochemical properties. Multiple sequence alignment (MSA) showed the presence of conserved catalytic triad S157, D254, H284. Maximum parsimony method using MEGA-6 distinguished the mesophilic and th.. View More»
    DOI: 10.4172/jpb.1000398

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