Journal of Chromatography & Separation Techniques
Open Access
ISSN: 2157-7064
ISSN: 2157-7064
Takayuki Shibamoto
Davis, California 95616
Tanzania
Research Article
LC-MS Analysis of Low Molecular Weight Carbonyl Compounds as 2,4-Dinitrophenylhydrazones Using Negative Ion Mode Electronspray Ionization Mass Spectrometry
Author(s): Akio Yasuhara, Yuuka Tanaka, Miyuki Makishima, Shigeru Suzuki and Takayuki ShibamotoAkio Yasuhara, Yuuka Tanaka, Miyuki Makishima, Shigeru Suzuki and Takayuki Shibamoto
Organic compounds in the environment, such as pesticides, degrade into inorganics via transformation into carbonyl compounds, such as formaldehyde, acetaldehyde, and acetone. 2, 4-Dinitrophenylhydrazones of 37 carbonyl compounds were prepared with 2, 4-dinitrophenyl hydrazine (DNPH). Their ESI negative spectra and EI positive spectra were obtained using an HPLC-mass spectrograph. EI positive spectra showed more ions than ESI negative spectra except in the case of two hydrazones. Molecular ions (M+) of carbonyl-DNPHs appeared as a base peak in the EI positive spectra in the case of all but 8 hydrazones. Many significant ions for the identification of a specific carbonyl compound are obtained by ESI negative spectra. Analysis of acetone in various waste waters was successfully conducted using a newly developed ESI negative spectroscopy method-levels found in the samples ranged from 2.1 .. View More»
DOI:
10.4172/2157-7064.1000108