Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
+44 1223 790975
ISSN: 0974-276X
+44 1223 790975
Shyh-Horng Chiou
Quantitative Proteomics Center and Graduate Institute of Medicine,
Kaohsiung
People's Republic of China
Research Article
Shotgun Proteomics Analysis of Differentially Expressed Urinary Proteins
Involved in the Hepatocellular Carcinoma
Author(s): Chao-Jen Kuo, Shen-Nien Wang, Shih-Shin Liang, Shu-Wen Chi, Zong Jing Yu, Edward Hsi, King-Teh Lee and Shyh-Horng ChiouChao-Jen Kuo, Shen-Nien Wang, Shih-Shin Liang, Shu-Wen Chi, Zong Jing Yu, Edward Hsi, King-Teh Lee and Shyh-Horng Chiou
Numerous investigations underlying the hepatocellular carcinoma (HCC) diagnosis as well as detection at an early stage based on hepatitis B virus surface antigen (HbsAg) concentration in serum and aflatoxin metabolites in urine have been commonly reported in the literature. To date, these biomarkers, even though specific and accurate, are not universal for the detection of HCC elicited by all causative factors. In addition, potential biomarkers may be present at low concentrations in contrast to the presence of abundant interfering proteins with a wide dynamic range. The aim of this study is to establish an operational analysis platform of effective and noninvasive diagnostic tool with a high sensitivity to explore protein expression profiles by shotgun proteomics using nano-liquid chromatography coupled tandem mass spectrometry (nanoLC-MS/MS) and stable isotope dimethyl labeling. Dif.. View More»
DOI:
10.4172/jpb.1000300