Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
+44 1223 790975
ISSN: 0974-276X
+44 1223 790975
Shui-Tein Chen
Room 709, Institute of Biological Chemistry, Academia Sinica 128, Academia Road Sec. 2,
Nankang, Taipei 115
People's Republic of China
Research Article
Proteomics Characterization of Haptoglobin Alpha-2 Subunit in Non-Small Cell Lung Cancer Serum and Various Human Materials
Author(s): Haou-Tzong Ma, Supawadee Sriyam, Supachok Sinchaikul, Hsien-Yu Tsai, Suree Phutrakul and Shui-Tein ChenHaou-Tzong Ma, Supawadee Sriyam, Supachok Sinchaikul, Hsien-Yu Tsai, Suree Phutrakul and Shui-Tein Chen
Non-small cell lung cancer (NSCLC) is detected in all lung cancer patients and the serum samples as potential source are often used as a potential source for early diagnosis. In this study, we used the proteomic approaches combining 2-DE, 2-D DIGE and mass spectrometry to identify the potential serum biomarkers in NSCLC serum samples, and compare the result to normal serum samples. The haptoglobin alpha 2 subunit (HAP2) is one of the proteins we are interested in and showed the overexpression level in NSCLC serum amongst the NSCLC subtypes. The identity of HAP2 was confirmed by LC-MS/MS and NH2-terminal amino acid sequencing analyses, while the high expression level of HAP2 in NSCLC serum samples was validated using co-immunoprecipitation and western blotting. Interestingly, the high expression of HAP2 has specificity in human serum samples, but nonspecific expression in lung cancer t.. View More»
DOI:
10.4172/jpb.1000280