Journal of Proteomics & Bioinformatics

Journal of Proteomics & Bioinformatics
Open Access

ISSN: 0974-276X

+44 1223 790975

Shufang Liang

Shufang Liang
West China Hospital,
1# Keyuan Road 4, Gaopeng Street, Chengdu 610041
China

Publications
  • Research Article
    Development of Deuterated-Leucine Labeling with Immunoprecipitation to Analyze Cellular Protein Complex
    Author(s): Shufang Liang, Xuejiao Xu, Haojie Lu and Pengyuan YangShufang Liang, Xuejiao Xu, Haojie Lu and Pengyuan Yang

    The deuterated-leucine (Leu-d 3 ) labeling is one kind of stable isotope labeling by amino acids in cell culture (SILAC), which has been widely used to compar e and quantify pr otein r elative expr ession. W e expanded an integrated immunoprecipitation (IP) coupled with SILAC approach (SILAC-IP) to differentiate the specific bind- ing par tners associated with a bait pr otein in two populations of cells. By this SILAC-IP strategy , the identified specific-binding proteins were quantified by tracking pairs of Leu-d 3 labeled and unlabeled peptides from the mass spectra, which could differentiate specific-binding proteins from nonspecific partners in high confidence. W e applied SILAC-IP method to dif fer entiate specific-binding pr oteins associated with 14-3-3â in human hepato- cellular carcinoma cel.. View More»
    DOI: 10.4172/jpb.1000037

    Abstract PDF

Relevant Topics

Top