Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
+44 1223 790975
ISSN: 0974-276X
+44 1223 790975
Sharma Neha
Dr Y S Parmar University of Horticulture and Forestry,
Solan 173230, (HP)
India
Research Article
Comparative In silico Analysis of Partial Coat Protein Gene Sequence of Zucchini Yellow Mosaic Virus Infecting Summer Squash (Cucurbita pepo L.) Isolated From India
Author(s): Sharma Neha, Bhardwaj Satya Vrat, Jarial Kumud, Thakur P D, Kaur Rajinder and Handa AnilSharma Neha, Bhardwaj Satya Vrat, Jarial Kumud, Thakur P D, Kaur Rajinder and Handa Anil
Zucchini yellow mosaic virus (ZYMV; Family: Potyviridae, Genus: Potyvirus) is a serious virus infecting summer squash causing severe damage to both crop and ornamental cucurbit crop production. In the present study, molecular characterization of ZYMV (at genomic and proteomic level) infecting summer squash was carried out and cDNA of approximately 700bp was amplified. The PCR amplified product was further sequenced and analyzed. The sequence of partial coat protein of 154 nucleotides of Indian isolate of zucchini yellow mosaic virus (ZYMV) was determined and translated to proteins. Later, the sequence was submitted to NCBI and has got accession no. GU144796 with protein id ACZ36948. In BLASTN analysis, nucleotide test sequence showed 91% homology with D13914 (sequence from USA), whereas, protein test sequence was 75.9% homologous in BLASTP analysis with a number of protein sequences p.. View More»
DOI:
10.4172/jpb.1000169