Journal of Chromatography & Separation Techniques
Open Access
ISSN: 2157-7064
+44 1300 500008
ISSN: 2157-7064
+44 1300 500008
Shabana Naz Shah
Pakistan
Research Article
Liquid Chromatographic Analysis of Prazosin in API, Dosage Form and Serum: Application to Drug-Metal Interaction Studies
Author(s): Najma Sultana, Muhammad Saeed Arayne and Shabana Naz ShahNajma Sultana, Muhammad Saeed Arayne and Shabana Naz Shah
Prazosin hydrochloride is a sympatholytic alpha-adrenergic blocker used in the treatment of anxiety, hypertension, refractory pulmonary oedema and panic disorders. Rapid, efficient, cost effective and reproducible isocratic reversed phase method has been developed and validated for the determination of prazosin in active pharmaceutical ingredient, dosage formulation and human serum using mobile phase 75:25 v/v acetonitrile:water having pH 3.20 adjusted with glacial acetic acid. Mobile phase was pumped at a flow rate of 1.5 mL min−1 using gradient elution through prepacked Nuclosil, C18 (250×4.6 mm, 10 μm) column. UV detection was performed at 250 nm. Method was validated following the ICH guidelines. Calibration curve was linear in concentration range 1.0-10 μg mL−1 with correlation coefficient 0.9999, and lower limits of detection and quantitation as 3.3 and 2.. View More»
DOI:
10.4172/2157-7064.1000197