Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
ISSN: 0974-276X
Satya Vrat Bhardwaj
India
Research Article
Inference on Coat Protein Evolution of Lily Symptomless Carlavirus in India and Abroad Based on Motifs Study and Phylogenetic Analysis
Author(s): Suhasini Huddone, Satya Vrat Bhardwaj, Rameshwar Singh Rattan, Kamlesh Kanwar, Gaurav Zinta and Anil HandaSuhasini Huddone, Satya Vrat Bhardwaj, Rameshwar Singh Rattan, Kamlesh Kanwar, Gaurav Zinta and Anil Handa
Lily symptomless carlavirus (LSV), the most common lily infecting virus around the world, contains 6 open reading frames (ORFs) in its genome, of which ORF5 representing coat protein (CP) is the most variable region and is used here to deduce phylogeny of the virus. CP gene of one of the LSV isolates present in the region, LSV isolate-Oh (Accession no. AJ748277) was taken as test sequence.Multiple sequence alignment of test sequence with ClustalW showed nucleotide and amino acid homology of up to 17-98% and 1-98%, respectively with other 78 carlaviral sequences from India and abroad. One conserved nucleotide motif of carlaviruses, AATAAA (Polyadenylation signal motif) was searched for, in the multiple sequence alignments but it was not found in any of the LSV isolates under study. Further, phylogenetic analysis of nucleotide sequences by DNADIST metho.. Read More»
DOI:
10.4172/jpb.1000141
