Journal of Chromatography & Separation Techniques
Open Access
ISSN: 2157-7064
ISSN: 2157-7064
Saeeda Nadir Ali
Karachi-75270
Pakistan
Research Article
Ultra Sensitive Liquid Chromatographic Method for the Simultaneous Determination of Carbamazepine with Nsaids in API, Pharmaceutical Formulation and Human Serum by Programming the Detector: Application to In Vitro Drug Interaction
Author(s): Najma Sultana, M. Saeed Arayne and Saeeda Nadir Ali
Najma Sultana, M. Saeed Arayne and Saeeda Nadir Ali
Here we describe an ultra-sensitive reverse phase liquid chromatographic method for the simultaneous determination of carbamazepine with NSAIDs in API, pharmaceutical formulation and human serum with UV detection. The separation of these multiple drug components was made by a Bondapak C18 column (10 μm, 25 cm×0.46 cm) using a moderate acidic mixture of mobile phase i.e., methanol:water 80:20 with pH adjusted to 3.0 using 85% o-phosphoric acid. The flow rate of the mobile phase was affixed at 1.0 ml min-1 for the best minimum interval of elusion of all the analytes. The detector response was monitored at isobestic point of 220 nm at ambient temperature. Calibration curves were obtained in the range of 0.4-12 μg mL-1 for carbamazepine, 0.5-16 μg mL-1 for meloxicam and 0.25-8.0 μg mL-1 for ibuprofen and mefenamic acid. Limits of detection were found to be 4.0, 3.0, 1.0,.. View More»
DOI:
10.4172/2157-7064.1000156