Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
+44 1223 790975
ISSN: 0974-276X
+44 1223 790975
Roger S Holmes
The Eskitis Institute for Drug Discovery and School of Natural Sciences,
Nathan 4111 QLD
Australia
Research Article
Comparative and Evolutionary Studies of Vertebrate Arylsulfatase B,
Arylsulfatase I and Arylsulfatase J Genes and Proteins: Evidence for an
ARSB-like Sub-family
Author(s): Roger S HolmesRoger S Holmes
Multiple sulfatase genes have been reported on the human genome, including Arylsulfatase B (ARSB), Arylsulfatase I (ARSI) and Arylsulfatase J (ARSJ). ARSB is localized in lysosomes and catalyses the hydrolysis of chondroitin and dermatan sulfate groups. Bioinformatic analyses of vertebrate genomes were undertaken using known human ARSB, ARSI and ARSJ amino acid sequences to study the relatedness and evolution of these genes and proteins. Several domain regions and key residues were conserved including signal peptides, active site residues, metal (Ca2+) and substrate binding sequences, disulfide linkages and N-glycosylation sites. The genes were widely expressed in human tissues with highest levels in esophagus (ARSB), lung (ARSI) and fibroblast cells (ARSB). Human ARSB was larger in size (>200 kb) and contained 8 coding exons, whereas ARSI and ARSJ contained only 2 coding exons amo.. View More»
DOI:
10.4172/jpb.1000418