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Journal of Proteomics & Bioinformatics

Journal of Proteomics & Bioinformatics
Open Access

ISSN: 0974-276X

Robyn L Ward

Robyn L Ward

Australia

Publications
  • Research Article
    Liquid Chromatography Tandem Mass Spectrometry for the Measurement of Global DNA Methylation and Hydroxymethylation
    Author(s): Jia Liu, Luke B Hesson and Robyn L WardJia Liu, Luke B Hesson and Robyn L Ward

    DNA methylation is an important epigenetic phenomenon. Global DNA methylation levels in the genome are tissue-specific and are reduced in the context of specific developmental and pathological states. Hence accurate assays to quantify global DNA methylation are required. In this review we describe different methods for global DNA methylation measurement and explain the methodology, strengths and limitations of the gold standard assay liquid chromatography tandem mass spectrometry (LC-MS/MS) for 5-methylcytosine and 5-hydroxymethylcytosine quantitation. Measurement of global DNA methylation using LC-MS/MS has helped to elucidate the effects of environmental exposures and demethylating drugs that can alter DNA methylation levels and further studies using this technique may provide insights on the prevention and/or reversal of DNA hypomethylation... Read More»
    DOI: 10.4172/jpb.S2-005

    Abstract PDF

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