Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
+44 1223 790975
ISSN: 0974-276X
+44 1223 790975
Robert A Hataway
Birmingham, AL 35229-2234
Tanzania
Research Article
Characterization of the Venom Proteome for the Wandering Spider,
Ctenus hibernalis (Aranea: Ctenidae)
Author(s): Jeffrey Cole, Patrick A Buszka, James A Mobley and Robert A HatawayJeffrey Cole, Patrick A Buszka, James A Mobley and Robert A Hataway
Spider venom is a rich multicomponent mixture of neurotoxic polypeptides. The venom of a small percentage of the currently classified spiders has been categorized. In order to determine what venom proteins are expressed in our species, the wandering spider Ctenus hibernalis, we constructed a comprehensive proteome derived from a crude venom extract using a GeLC approach that required a one dimensional denatured gel electrophoresis separation combined with enzymatic digestion of the entire lane cut into many molecular weight fractions followed by LC-ESI-MS2. In this way, we identified 1,182 proteins with >99% confidence that closely matched sequences derived from the combined genomes taken from several similar species of spiders. Our results suggest that the venom proteins of C. hibernalis contain several proteins with conserved sequences similar to other species. Going forward, wit.. View More»
DOI:
10.4172/jpb.1000406