Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
+44 1223 790975
ISSN: 0974-276X
+44 1223 790975
Ram Singh Purty
University School of Biotechnology, Guru Gobind Singh Indraprastha University,
New Delhi
India
Research Article
Structural and Expression Analysis of Salinity Stress Responsive Phosphoserine Phosphatase from Brassica juncea (L.)
Author(s): Ram Singh Purty, Madhav Sachar and Sayan ChatterjeeRam Singh Purty, Madhav Sachar and Sayan Chatterjee
Plants exposed to environment generally encounter various abiotic stresses which include drought, salinity, mineral deficiency, high and low temperature leading to reduction in growth and productivity. Phosphoserine phosphatase (EC 3.1.3.3) belongs to Haloacid Dehalogenase (HAD) superfamily and it catalyzes the last step in the phosphorylated pathway for the biosynthesis of L-Ser. In the present investigation, the full-length cDNA coding for phosphoserine phosphatase was cloned and purified from Brassica juncea L. Sequence analysis indicates that it encodes a protein of 296 amino acids and revealed high homology to PSP of Arabidopsis thaliana. Multiple sequence alignment analysis showed the presences of the three short conserved sequence motifs. Molecular modelling of BjPSP showed core α/β domain of Rossmann fold which is the characteristic of members of HAD superfamily. Wes.. View More»
DOI:
10.4172/jpb.1000432