Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
+44 1223 790975
ISSN: 0974-276X
+44 1223 790975
Quxiao Du
People’s Hospital of Zhengzhou,
No. 33 Huanghe Road, Zhengzhou, 450003
China
Research Article
Study on the Relationship between the Structure and Functions of Antihuman Cervical Cancer Single-chain Antibody and the Lengths of Linkers
Author(s): Jie Cheng, Quxiao Du, Xiaoli Zhang, Yafeng Ren, Boai Zhang and Xiaodong FengJie Cheng, Quxiao Du, Xiaoli Zhang, Yafeng Ren, Boai Zhang and Xiaodong Feng
Background: This study aimed to find the linker length with minimal impact among chains to fight against the structure and function of cervical cancer single-chain antibody.
Methods: The original variable region of heavy chain (VH) and variable region of light chain (VL), and the singlechain antibody with (Gly4Ser)n linkers of different lengths (n=1~8) were modeled using SWISS-MODEL homologous modeling. Comparison of the similarity of original VH/VL and VHn/VLn was carried out by applying the algorithm of spatial hierarchical alignment based on the spherical coordinates. The fore and aft distance and diffusion radius of α were also calculated. The stability of antibody with different linker length was then compared. ELISA method was adopted to evaluate the immunological activity of single-chain antibody with different linkers. MTT as.. View More»
DOI:
10.4172/jpb.S8-004