Journal of Chromatography & Separation Techniques

Journal of Chromatography & Separation Techniques
Open Access

ISSN: 2157-7064

+44 1300 500008

Prosser RS

Prosser RS

Canada

Publications
  • Research Article
    Notes on Synthesis of Perdeutero-5-13C,5,5,5-Trifluoroisoleucine VI56
    Author(s): Naugler DG and Prosser RS Naugler DG and Prosser RS

    The 13CF3 group is a promising label for heteronuclear (19F,13C) NMR studies of proteins. Desirable locations for this NMR spin label include the branched chain amino acid methyl groups. It is known that replacement of CH3 by CF3 at such locations preserves protein structure and function and enhances stability. 13CF3 may be introduced at the δ position of isoleucine and incorporated biosynthetically in highly deuterated proteins. This paper reports our work in synthesis and purification of 5,5,5-trifluoroisoleucine, its perdeutero and 5-13C versions and of 2-13C-trifluoroacetate and its utility as a precursor for introduction of the 13CF3 group into proteins. .. View More»
    DOI: 10.4172/2157-7064.1000375

    Abstract PDF

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