Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
ISSN: 0974-276X
Pham Duc Dan
Vietnam
Research Article
Analysis of the Membrane Proteins in Human Serum
Author(s): Pham Duc Dan, Tran Thai Thuong, Pham Dinh Minh, Do Doan Loi, Nguyen Bich Nhi and Phan Van ChiPham Duc Dan, Tran Thai Thuong, Pham Dinh Minh, Do Doan Loi, Nguyen Bich Nhi and Phan Van Chi
Serum and membrane proteins are two of the most attractive targets for proteomic analysis. Previous membrane protein studies tend to focus on tissue samples, while researches on membrane proteins in serum are still limited. In this study, human albumin and IgG depleted sera have been used for the proteome separation by SDS-PAGE. After staining, gels with protein fractions were cut and digested by trypsin. The peptide mixtures extracted from each gel slice were analyzed by nano-liquid chromatography-electrospray ionization mass spectrometry (NanoLCESI- MS/MS). The proteins were identified by Mascot v1.8 software and validated by MSQuant v1.5 software. As the result, a database including 1,216 membrane proteins, of which 469 (38.6%) membrane proteins with at least one transmembrane domain (TMD) were identified based on three different transmembrane prediction applications (Phobius, TMHM.. View More»
DOI:
10.4172/jpb.1000294