Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
+44 1223 790975
ISSN: 0974-276X
+44 1223 790975
P Dolashka
Institute of Organic Chemistry with Centrum of Phytochemistry, Bulgarian Academy of Sciences,
G. Bonchev 9, 1113 Sofia
Bulgaria
Research Article
Proteomic Analyses of Exothermic Processes in Rat Brain Homogenate
Author(s): Y Voynikov, L Velkova, L Tancheva, P Mladenov, A Dolashki, L Alova, W Voelter and P DolashkaY Voynikov, L Velkova, L Tancheva, P Mladenov, A Dolashki, L Alova, W Voelter and P Dolashka
Alzheimer’s disease (AD) is the most widespread neurodegenerative disorder which can be induced by scopolamine, but the underlying molecular mechanism is poorly understood. Recently, differential scanning calorimetry (DSC) has been used to study healthy and scopolamine-treated mice. A well-expressed exothermic transition minimum in the range of 35 - 45°C was determined in the DSC profiles of healthy mice supernatants. To explain this process, using two-dimensional gel electrophoresis (2D-PAGE) coupled with MALDI-TOF-TOF, poorly soluble membrane proteins in hippocampal proteome of rat brain tissue were identified. The different behavior of the hippocampal proteome from the healthy rats before and after heating to 45oC was identified. Due to the demonstrated change in protein level of tau protein and tubulin in the rat hippocampus after heating to 45°C, it was suggested th.. View More»
DOI:
10.4172/jpb.1000470