Journal of Proteomics & Bioinformatics

Journal of Proteomics & Bioinformatics
Open Access

ISSN: 0974-276X

+44 1223 790975

NC Rath

NC Rath

Fayetteville, AR 72701
Tanzania

Publications
  • Research Article
    Identification and Structural Characterization of Avian Beta-Defensin 2 Peptides from Pheasant and Quail
    Author(s): L Kannan, R Liyanage, JO Lay Jr., B Packialakshmi, NB Anthony and NC Rath L Kannan, R Liyanage, JO Lay Jr., B Packialakshmi, NB Anthony and NC Rath

    Pheasant and quail orthologs of Avian β-Defensin 2 (AvBD2) were identified by screening heterophil extracts using Matrix-Assisted Laser Desorption Ionization Time-of-Flight (MALDI-TOF) Mass Spectrometry (MS), comparative profiling, chemical, and bioinformatic characterizations. In heterophil extracts of each pheasant and quail, we observed a single high intensity mass peak, corresponding to m/z 4114.8 and 4163.8, respectively which upon reduction and alkylation were shifted by a mass difference of 348 Da indicative of the modification of 3 internal disulfide bonds that exist in these peptides. The unmodified peptides, m/z 4114 and 4163, and their Carbamidomethylated (CAM) derivatives were purified by reverse phase HPLC. Using the purified peptides we determined their partial sequences by trypsin digestion followed by MALDI-TOF-MS.. View More»
    DOI: 10.4172/jpb.1000258

    Abstract PDF

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