Journal of Chromatography & Separation Techniques
Open Access
ISSN: 2157-7064
ISSN: 2157-7064
Naresh Kumar Katari
Hyderabad, Telangana
India
Research Article
Development and Validation of RP-HPLC Method for Simultaneous
Determination of Diclofenac Potassium and its Process Related Impurities in
Solid Oral Dosage Form
Author(s): Thirupathi Dongala, Ashok Kumar Palakurthi, Kiran Kumar Velaveni and Naresh Kumar KatariThirupathi Dongala, Ashok Kumar Palakurthi, Kiran Kumar Velaveni and Naresh Kumar Katari
The paper reports an efficient and rapid fast LC method for estimation of diclofenac potassium and its impurities. The main aim of the study is to develop and validate a simple, rapid, accurate, sensitive, less time consuming and less expensive method by using RP-HPLC with UV detector. The chromatographic separation of diclofenac potassium and its impurities was carried out by using 150 × 4.6 mm, i.d., 5 μm C-18 column with prepared mobile phase-A consisting 800:200 (v/v) of 0.01 M ammonium acetate adjusted pH 5.3 with acetic acid and acetonitrile and mobile phase-B consisting 200:800 (v/v) of 0.01 M ammonium acetate adjusted pH 5.3 with acetic acid and acetonitrile. The wavelength for detection of were made at 280.0 nm using UV detector. The flow rate of 1 mL/min. The method was able to detect and separate impurities and diclofenac potassium. The system suitability parameter.. View More»
DOI:
10.4172/2157-7064.1000412