Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
ISSN: 0974-276X
Muhammad Asif Nawaz
Pakistan
Research Article
A Novel Methodology for Structural, Functional and Toxicological Analysis of Mutant Angiogenin Protein in Human
Author(s): Muhammad Aamir Hassan, Aqib Zafar Khan, Basit Haidar, Muhammad Asif Nawaz, Rabbia Mumtaz and Simon ManzoorMuhammad Aamir Hassan, Aqib Zafar Khan, Basit Haidar, Muhammad Asif Nawaz, Rabbia Mumtaz and Simon Manzoor
Introduction: Angiogenin is a protein of 14.1 kDa encoded by ANG gene and belongs to a superfamily of pancreatic ribonuclease A. Angiogenin is an effective stimulator of new blood vessels formation. It plays a vital role in the pathological as well as physiological mechanisms by regulating cell proliferation, differentiation and invasion. Mutation in ANG leads to a disease called amyotrophic lateral sclerosis 9. Amyotrophic lateral sclerosis 9 is a motor neuron disease that causes the decease of neurons, which controls the voluntary muscles of body.
Material and Methods: The mutations F12S, P20S, Q36L, Y38H, K41E, D46G, S52N, R55K, C63W, K64I, I70V, K84E, P136L, V137I and H138R were selected for this study to investigate the single amino acid substitution effects on structure, function, stability and pathological impression on the protein... View More»
DOI:
10.4172/jpb.1000450