Journal of Chromatography & Separation Techniques
Open Access
ISSN: 2157-7064
ISSN: 2157-7064
Moon Ik Chang
Republic of Korea
Research Article
Development of an Improved HPLC-PDA Method for the Determination of Tolfenamic Acid in Meat and Milk
Author(s): Ji-Sun Lee, Soo Hee Cho, Chae Mi Lim, Moon Ik Chang and Hojae BJi-Sun Lee, Soo Hee Cho, Chae Mi Lim, Moon Ik Chang and Hojae B
Tolfenamic acid (TA) is one of the non-steroidal anti-inflammatory drugs (NSAIDs) which are widely used as analgesic, anti-inflammatory and antipyretic drugs. In this study, we developed an improved detection method focusing on the validation of the TA residue using high performance liquid chromatography (HPLC) and a photodiode array (PDA) detector. TA in bovine, swine muscles and milk were analyzed using a C18 column (5 μm, 250 mm × 4.6 mm i.d.) and the mobile phase consisted of water with 0.1% phosphoric acid (eluent A) and 0.1% phosphoric acid in acetonitrile (ACN) (eluent B). Additionally, to optimize TA detection, liquid-liquid extraction (LLE) and additional purification steps were established to minimize the endogenous peaks and interferences. The developed method was validated through the limit of detection (LOD), the limit of quantification (LOQ), linearity, accuracy.. View More»
DOI:
10.4172/2157-7064.1000381