Journal of Proteomics & Bioinformatics
Open Access

Mike Whalley

Publications

• Short Communication
  A Free Flow Electrophoresis Separation Strategy for Segregation of High Abundant Phycobilisomes from Cyanobacterium Nostoc punctiforme PCC 73102
  Author(s): Nishikant V Wase, Saw Yen Ow, Malinda Salim, Mikkel Nissum, Mike Whalley and Phillip C WrightNishikant V Wase, Saw Yen Ow, Malinda Salim, Mikkel Nissum, Mike Whalley and Phillip C Wright
  
  Generally proteomics analysis of cyanobacteria has limited dynamic resolution because of the presence of abundant proteins. Thus proteomics assessment of the low abundance proteins in cyanobacteria is difficult as it comprises phycobilisome and Rubisco. The current investigation assesses the performance of FFE in isoelectric focusing (IEF) mode: a solution-based technique that separates proteins on the basis of isoelectric point (pI). We explore the advantages of combining robust protein fractionation using FFE and 1D-LC-MS/MS to extend protein identification deeper through the dynamic range of the model cyanobacterium, Nostoc punctiforme PCC 73102. Sixty-one novel new proteins (out of 248 (all identified with ≥ 2 peptides)) were successfully identified using FFE-IEF compared to all previous reports. Results demonstrate the ability of FFE to provide improved protein distribution.. View More»
  DOI: 10.4172/jpb.1000215

  Abstract PDF

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