Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
ISSN: 0974-276X
Meera Srivastava
Physiology and Genetics, USU School of Medicine,
4301 Jones Bridge Road, Bethesda. MD, 20814
Tanzania
Research Article
Reduced PARP1 as a Serum Biomarker for Graft Rejection in Kidney
Transplantation
Author(s): Meera Srivastava, Yelizaveta Torosyan, Ofer Eidelman, Catherine Jozwik, Harvey B. Pollard and Rosyln MannonMeera Srivastava, Yelizaveta Torosyan, Ofer Eidelman, Catherine Jozwik, Harvey B. Pollard and Rosyln Mannon
A serum proteomics platform enabling expression profiling in transplantation-associated clinical subsets gives an opportunity to identify non-invasive biomarkers that can accurately predict transplant outcome. In this study, we attempted to identify candidate serum biomarkers that could predict kidney allograft rejection/injury, regardless of its etiological and therapeutic heterogeneity. Using serum samples collected from kidney transplantation patients and healthy controls, we first employed Clontech-500 Ab microarrays to profile acute rejection (AR) and chronic graft injury (CGI) versus stable graft function (SF) and normal kidneys (NK). Using GenePattern analysis of duplicate arrays on pooled samples, we identified gender-independent biomarkers PARP1, MAPK1, SRP54, DP1, and p57 (FDR ≈ 25%), the concordant downregulation of which represented a detrimental profile common for b.. View More»
DOI:
10.4172/jpb.1000350