Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
+44 1223 790975
ISSN: 0974-276X
+44 1223 790975
Masaoki Takano
School of Pharmaceutical Sciences,
1-1-3 Minatojima, Chuo-ku, Kobe 650-8586
Japan
Research Article
Secretomic Analysis of Mouse Choroid Plexus Cell Line ECPC-4 Using
Two-Dimensional Gel Electrophoresis Coupled to Mass Spectrometry
Author(s): Masaoki Takano, Mari Uramoto, Mieko Otani, Kenji Matsuura, Keiji Sano and Shogo MatsuyamaMasaoki Takano, Mari Uramoto, Mieko Otani, Kenji Matsuura, Keiji Sano and Shogo Matsuyama
Choroid plexus (CP), the main production site of cerebrospinal fluid (CSF), is responsible for the inflammatory mediators involved in meningitis and immune systems. The mouse choroid plexus cell line ECPC-4 is known to be useful for analyses of CP functions. In this study, we performed the secretome analysis of ECPC-4 using two-dimensional gel electrophoresis (2-DE) followed by mass spectrometry. Twenty-two secreted proteins were identified in the conditioned medium of ECPC-4. They were a secreted protein acidic and rich in cysteine (SPARC), plasminogen activator inhibitor 1 (PAI-1), and others (vinculin, heat shock protein cognate 71 kDa, moesin, tubulin ß2A, fascin, calreticulin, gamma actin, alcohol dehydrogenase, malate dehydrogenase, Rho GDP dissociation inhibitor alpha, phosphoglyceratemutase 1, translationally controlled tumor protein, phosphatidylethanolamin binding prote.. View More»
DOI:
10.4172/jpb.1000338