Journal of Chromatography & Separation Techniques
Open Access
ISSN: 2157-7064
+44 1300 500008
ISSN: 2157-7064
+44 1300 500008
María de los Ángeles Colín- Cruz
Carretera Toluca-Ixtlahuaca Km. 15, El Cerrillo Piedras Blancas, C.P.50200 Toluca
Research Article
Optimization of Headspace Solid-Phase Microextraction and Gas
Chromatography Coupled with Mass Spectrometry Technique for the
Quantification of Volatile Compounds in a Fresh Cheese (Oaxaca)
Author(s): José Rogelio Sandoval- Copado, Juan Orozco- Villafuerte, María Magdalena García- Fabila and María de los Ángeles Colín- CruzJosé Rogelio Sandoval- Copado, Juan Orozco- Villafuerte, María Magdalena García- Fabila and María de los Ángeles Colín- Cruz
The Head Space Solid Phase Microextraction Method (HS-SPME), coupled with Gas Chromatography and Mass Spectrometry (GC-MS) was optimized to quantify Volatile Organic Compounds (VOC’s) in a fresh Oaxaca cheese during its shelf life of 22 days. Optimal extraction conditions were ascertained by means of a multiple linear regression analysis and the generation of a response surface. Conditions to reach the equilibrium of VOC’s at the headspace were established to be 30 minutes of ultrasonic agitation at 50°C. The conditions for extraction were exposure of the fiber at room temperature for 30 minutes. Optimal extraction of volatile compounds released by the Oaxaca cheese allowed identification of 21 volatile compounds: 8 acids, 1 alcohol, 4 aldehydes, 4 ketones, 1 ester, and 3 lactones. Quantification of VOC’s was carried out using the technique of standard addition. .. View More»
DOI:
10.4172/2157-7064.1000415