Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
+44 1223 790975
ISSN: 0974-276X
+44 1223 790975
Magda Spella
Greece
Research Article
P68/ Ddx5 RNA Helicase Interacts and Co-Localizes In Vivo with De Novo the DNA Methyltransferases Dnmt3a1 and Dnmt3a2
Author(s): Anastasia Mpakali, Andriana G. Kotini, Magda Spella, Marina Kouyialis, Angelliki Tserga, Leonidas Fragkos-Livanios, Martina Samiotaki and Theodora AgaliotiAnastasia Mpakali, Andriana G. Kotini, Magda Spella, Marina Kouyialis, Angelliki Tserga, Leonidas Fragkos-Livanios, Martina Samiotaki and Theodora Agalioti
The 5-methyl cytosine (5meC) genomic methylation patterns play crucial roles in mammalian development and are altered in cancer. The enzymes that create, maintain and modify the DNA methylation patterns are the DNA methyltransferases (Dnmts) which are all encoded by essential genes. The de novo Dnmts -Dnmt3a and Dnmt3b- establish the DNA methylation patterns early in mammalian development by introducing DNA methylation marks where no previous methylation exists. These enzymes do not exhibit affinity for specific DNA sequences, thus their recruitment to specific DNA loci and their activities must be tightly regulated. In particular, Dnmt3a2 –one of the two protein isoforms produced by the Dnmt3a locus- is the most abundant DNA methyltransferase in mouse Embryonic Stem Cells. To identify Dnmt3a (and DNA methylation) regulators we have searched for Dnmt3a2 interacting proteins in m.. View More»
DOI:
10.4172/jpb.1000207