Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
+44 1223 790975
ISSN: 0974-276X
+44 1223 790975
Luc Guerrier
France
Research Article
Protein Sample Treatment with Peptide Ligand Library: Coverage and Consistency
Author(s): Lei Li, ChengJun Sun, Steve Freeby, Dennis Yee, Sylvie Kieffer-Jaquinod, Luc Guerrier, Egisto Boschetti and Lee LomasLei Li, ChengJun Sun, Steve Freeby, Dennis Yee, Sylvie Kieffer-Jaquinod, Luc Guerrier, Egisto Boschetti and Lee Lomas
Low-abundance protein detection in biological samples is one of the main challenges in proteomics investigations. One approach that makes the detection of rare species possible is the treatment of biological samples with solidphase combinatorial peptide ligand libraries. However, the use of combinations of ligands opens an uncertainty in that, since the diversity of the library is very large, aliquots of beads sampled from the library might not have fully comparable bead species each time. Reproducibility of experimental data with highly diverse libraries is therefore a main concern to address. This paper reports reproducibility data when aliquots of similar and different volumes of libraries are used at a certain sample to library ratio. Eluates from ligand libraries and other fractions are analyzed using various complementary methods such as two-dimensional gel elec.. View More»
DOI:
10.4172/jpb.1000110