Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
+44 1223 790975
ISSN: 0974-276X
+44 1223 790975
Lawrence J. Mandarino
Center for Metabolic and Vascular Biology,
P. O. Box 874501, Tempe AZ 85287-4501
Tanzania
Research Article
High Fat Diet-Induced Changes in Hepatic Protein Abundance in Mice
Author(s): Moulun Luo, April E. Mengos, Tianna M. Stubblefield and Lawrence J. MandarinoMoulun Luo, April E. Mengos, Tianna M. Stubblefield and Lawrence J. Mandarino
Nonalcoholic fatty liver disease (NAFLD) is associated with obesity, insulin resistance, type 2 diabetes, and dyslipidemia. The purpose of this study was to identify novel proteins and pathways that contribute to the pathogenesis and complications of NAFLD. C57BL/6J male mice were fed a 60% (HFD) or 10% (LFD) high or low fat diet. HFD induced obesity, hepatic steatosis and insulin resistance (euglycemic clamps, glucose infusion rate: LFD 50.5 ± 6.4 vs. HFD 14.2 ± 9.5 μg/ (g . min); n = 12). Liver proteins were analyzed by mass spectrometry-based proteomics analysis. Numerous hepatic proteins were altered in abundance after 60% HFD feeding. Nine down-regulated and nine up-regulated proteins were selected from this list for detailed analysis based on the criteria of 1.5-fold difference, consistency across replicates, and having at least 2 spectra assigned. Proteins that d.. View More»
DOI:
10.4172/jpb.1000214