Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
ISSN: 0974-276X
Jubilee Purkayastha
Office of the Director General-Life Sciences, Defence Research and Development Organization,
Rajaji Marg, New Delhi-110011
India
Research Article
World's Hottest Chilli Bhut Jolokia (Capsicum assamicum) Proteome
Revealed: Comparative Proteomic Analysis of Differentially Expressed
Proteins
Author(s): Jubilee Purkayastha, Syed Imteyaz Alam, Nabonita Sengupta, Bikash Nath, Bhoj Kumar, Hemanta Kumar Gogoi, Lokendra Singh and Vijay VeerJubilee Purkayastha, Syed Imteyaz Alam, Nabonita Sengupta, Bikash Nath, Bhoj Kumar, Hemanta Kumar Gogoi, Lokendra Singh and Vijay Veer
With proteomic analysis including 2-DE, image analysis, and protein identification with MALDI-TOF/MS, an investigation aiming at a enhanced understanding of the whole fruit proteome and differentially expressed proteins and/or gene products was carried out with total fruit extracts from Bhut Jolokia (Capsicum assamicum) and less pungent Capsicum frutescens. A total of 107 dominant protein spots have been identified here using a 2-DE/MS technique. Among the identified proteins, 14 proteins exhibited qualitative difference with unique expression in Bhut Jolokia and 6 proteins showed quantitative differential expression alterations. Among the 6 differential proteins, one was down-regulated and 5 were up-regulated. Apart from the unique and differential proteins for which a cluster of orthologous groups (COG) could not be assigned (36.84%), most of the dominant unique and differentially e.. View More»