Journal of Chromatography & Separation Techniques
Open Access
ISSN: 2157-7064
ISSN: 2157-7064
Joseph SR
India
Research Article
Simultaneous Determination of Paracetamol, Aceclofenac and Chlorzoxazone in Pharmaceutical Dosage Form by UHPLC Method
Author(s): Prema Kumari KB, Murugan V, Ezhilarasan V and Joseph SRPrema Kumari KB, Murugan V, Ezhilarasan V and Joseph SR
Aim: UHPLC method was developed and validated for the determination of Paracetamol, Aceclofenac and Chlorzoxazone in pharmaceutical dosage form. Methods: Separation was performed in Agilent technologies 1220 infinity LC system equipped with open lab CDS Chemstation software using Eclipse plus C18 (50 × 4.6 mm id, 1.8 μm) column with mobile phase containing 0.05% orthophosphoric acid, adjusted to pH 6.0 with triethylamine and acetonitrile in the ratio of 70:30 v/v with a flow rate of 0.5 mL/min and detection wavelength 270 nm. Results: The method was found to be linear in the concentration range of 161.59-484.77 μg/mL for paracetamol, 49.69-149.09 μg/mL for aceclofenac and 124.81-374.43 μg/mL for chlorzoxazone with correlation coefficient of 0.999 for all the three drugs respectively. The %RSD of 0.57, 0.45 and 0.60 for intra-day and 0.96, 0.88 and 0.84 for inter-day .. View More»
DOI:
10.4172/2157-7064.1000384