Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
ISSN: 0974-276X
Jonas S Almeida
Biomedical Informatics Department,
Tanzania
Review Article
Mechanistic Parameterization of the Kinomic Signal in Peptide Arrays
Author(s): Alex Dussaq, Joshua C Anderson, Christopher D Willey and Jonas S AlmeidaAlex Dussaq, Joshua C Anderson, Christopher D Willey and Jonas S Almeida
Kinases play a role in every cellular process involved in tumorigenesis ranging from proliferation, migration, and protein synthesis to DNA repair. While genetic sequencing has identified most kinases in the human genome, it does not describe the ‘kinome’ at the level of activity of kinases against their substrate targets. An attempt to address that limitation and give researchers a more direct view of cellular kinase activity is found in the PamGene PamChip® system, which records and compares the phosphorylation of 144 tyrosine or serine/threonine peptides as they are phosphorylated by cellular kinases. Accordingly, the kinetics of this time dependent kinomic signal needs to be well understood in order to transduce a parameter set into an accurate and meaningful mathematical model.
Here we report the analysis and mathematical modeling of kin.. View More»
DOI:
10.4172/jpb.1000401