Journal of Proteomics & Bioinformatics
Open Access

Johan T den Dunnen

Publications

• Research Article
  Serum Peptide Profiles of Duchenne Muscular Dystrophy (DMD) Patients Evaluated by Data Handling Strategies for High Resolution Content
  Author(s): Vishna D Nadarajah, Bart JA Mertens, Hans Dalebout, Marco R Bladergroen, Sharmini Alagaratnam, Penny Garrood, Kate Bushby, Volker Straub, André M Deelder, Johan T den Dunnen, Gert-Jan B van Ommen, Peter AC ‘t Hoen and Yuri EM van der BurgtVishna D Nadarajah, Bart JA Mertens, Hans Dalebout, Marco R Bladergroen, Sharmini Alagaratnam, Penny Garrood, Kate Bushby, Volker Straub, André M Deelder, Johan T den Dunnen, Gert-Jan B van Ommen, Peter AC ‘t Hoen and Yuri EM van der Burgt
  
  The accuracy of Mass Spectrometry (MS)-based analysis of peptides in complex biological mixtures improves upon using high resolution instrumentation. However, high resolution content poses challenges to data processing and statistical analysis. Here, three different data handling strategies were evaluated with respect to classification performance using a well-defined cohort of serum samples from Duchenne Muscular Dystrophy (DMD) patients and controls. For this purpose, serum samples were purified using a solid-phase extraction (SPE) protocol based on Reversed-Phase (RP) C18 magnetic beads. Isotopically-resolved peptide profiles were acquired on a Matrix-Assisted Laser Desorption/Ionization Time-of-Flight (MALDI-TOF) mass spectrometer and examined by either using the full mass spectrum or after selecting peaks between 1000<m/z<4000 followed by data filtering or data integration... View More»
  DOI: 10.4172/jpb.1000219

  Abstract PDF

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