Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
+44 1223 790975
ISSN: 0974-276X
+44 1223 790975
Ida Pucci-Minafra
Center of Experimental Oncobiology (C.OB.S), University of Palermo and La Maddalena Hospital,
Palermo
Italy
Research Article
Proteomic Profiling of In-Vitro Bone-Conditioned Skbr3 Breast Cancer
Cells
Author(s): Ida Pucci-Minafra, Gianluca Di Cara, Rosa Musso, Giovanni Peri, Biagio Valentino, Michele D\'Arienzo, Desiree Martini, Mario Raspanti and Salvatore MinafraIda Pucci-Minafra, Gianluca Di Cara, Rosa Musso, Giovanni Peri, Biagio Valentino, Michele D\'Arienzo, Desiree Martini, Mario Raspanti and Salvatore Minafra
A major complication of advanced breast cancer is the frequent formation of bone metastases. To our knowledge, however, the information on the phenotypic properties of cancer cells able to survive and colonize the bone tissue is still fragmentary. We report here a proteomic study of breast cancer cells (SKBR3) collected after a colonization of bone fragments in a special model of co-culture, used to simulate the phenotype of breast cancer metastasizing cells. The scanning electron microscopy of the seeded cancer cells into the bone fragments, showed their peculiar traits of multilayered growth and spiny surface, typical of a very aggressive phenotype. The comparative proteomic profiling of the bone-conditioned cells (SKBR3-B1) versus the parental cell line (SKBR3-WT), performed by applying two-dimensional electrophoresis (2D-IPG) and mass spectrometry (Maldi / TOF) revealed the existe.. View More»
DOI:
10.4172/jpb.1000392