Journal of Chromatography & Separation Techniques
Open Access
ISSN: 2157-7064
+44 1300 500008
ISSN: 2157-7064
+44 1300 500008
Hortas ML
Spain
Research Article
Development of COL3A1-Specific Real-Time PCR Assay for Clinical and Sport Detection Applications
Author(s): Martinez B, Jimenez A, Diaz T, Gómez JM, Hortas ML, Segura J, GarcÃa-Fuentes E and Ferro PMartinez B, Jimenez A, Diaz T, Gómez JM, Hortas ML, Segura J, García-Fuentes E and Ferro P
PIIINP, whose gene is COL3A1, is a protein used as a biomarker, which is altered in various pathologies such as fatty liver of non-alcoholic origin and is also used to detect abuse of human recombinant growth hormone (rhGH) by elite athletes to improve their performance. Therefore, the study of the levels of this protein or its gene could be useful for the diagnosis of these pathologies, in addition to other uses. The objective of this work was the development of a new detection method of the COL3A1 gene based on the quantification of mRNA in real time. 10 healthy men volunteers were injected intravenously for 3 consecutive days with 0.026 mg-1kg-1day-1person-1 of rhGH, and serum and blood samples were collected for 7 days at different times. mRNA extraction was performed after lymphocyte purification following standard protocols, and samples were quantified by quantitative real-time .. View More»
DOI:
10.4172/2157-7064.1000403