Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
+44 1223 790975
ISSN: 0974-276X
+44 1223 790975
Hongzhan Huang
Tanzania
Research Article
Integrated Bioinformatics for Radiation-Induced Pathway Analysis from Proteomics and Microarray Data
Author(s): Zhang-Zhi Hu, Hongzhan Huang, Amrita Cheema, Mira Jung, Anatoly Dritschilo and Cathy H. WuZhang-Zhi Hu, Hongzhan Huang, Amrita Cheema, Mira Jung, Anatoly Dritschilo and Cathy H. Wu
Functional analysis and interpretation of large-scale proteomics and gene expression data require effective use of bioinformatics tools and public knowledge resources coupled with expert-guided examination. An integrated bioinformatics approach was used to analyze cellular pathways in response to ionizing radiation. ATM, or mutated in ataxia-telangiectasia, a serine-threonine protein kinase, plays critical roles in radiation responses, including cell cycle arrest and DNA repair. We analyzed radiation responsive pathways based on 2D-gel/MS proteomics and microarray gene expression data from fibroblasts expressing wild type or mutant ATM gene. The analysis showed that metabolism was significantly affected by radiation in an ATM dependent manner. In particular, purine metabolic pathways were differentially changed in the two cell lines. The expression of ribonucleoside- .. View More»
DOI:
10.4172/jpb.1000009