Journal of Chromatography & Separation Techniques
Open Access
ISSN: 2157-7064
+44 1300 500008
ISSN: 2157-7064
+44 1300 500008
Haiyan Liu
College of Pharmacy,
Hebei Province Key Laboratory of Pharmaceutical Quality Control Baoding, 071002
China
Research Article
On-Line Solid-Phase Extraction Based on Poly (Nipaam-MAA-co-EDMA) Monolith Coupled with High-Performance Liquid Chromatography for Determination of Nitrendipine and Nisoldipine in Human Urine
Author(s): Yanhui Duan, Haiyan Liu, Suying Sun, Yanzhao Gu, Jia Li and Gengliang YangYanhui Duan, Haiyan Liu, Suying Sun, Yanzhao Gu, Jia Li and Gengliang Yang
A solid-phase extraction absorbent material based on poly (N-isopropylacrylamide-co- methacrylic acidco-ethylene glycol dimethacrylate) [poly (NIPAAm-MAA-co-EDMA)] monolithic column was developed for the simultaneous determination of nitrendipine and nisoldipine from human urine. The morphology of monolithic column and pressure drop across the columns were characterized. The results showed excellent permeability and high selectivity. Urine samples (0.05 mL) were injected directly onto the prepared Solid-Phase Extraction (SPE) monolithic column. Most biological matrix compounds in urine were removed by on-line SPE technology, while nitrendipine and nisoldipine retained on the SPE column were effectively separated on a C18 analytical column. For all analytes, linear calibration curves were obtained over a range of 50-500 ng/mL with coefficient of correlation > 0.9996. Accuracy and pr.. View More»
DOI:
10.4172/2157-7064.1000123