Journal of Chromatography & Separation Techniques
Open Access
ISSN: 2157-7064
+44 1300 500008
ISSN: 2157-7064
+44 1300 500008
Gabriella Roda
Dipartimento di Scienze Farmaceutiche, Università degli Studi di Milano,
Via Mangiagalli 25, 20133 Milano
Italy
Research Article
Quali-Quantitative Analysis by LC/DAD and GPC of the Polyphenols of “Uva Di Troia Canosina” Grape Seeds for the Development of an Industrial Nutraceutical Product
Author(s): Martina Bava, Sebastiano Arnoldi, Lucia Dell’Acqua, Sergio Fontana, Flavia La Forgia, Giuseppe Mustich, Gabriella Roda, Chiara Rusconi, Giovanni Sorrenti, Giacomo Luca Visconti and Veniero GambaroMartina Bava, Sebastiano Arnoldi, Lucia Dell’Acqua, Sergio Fontana, Flavia La Forgia, Giuseppe Mustich, Gabriella Roda, Chiara Rusconi, Giovanni Sorrenti, Giacomo Luca Visconti and Veniero Gambaro
The quali-quantitative determination of the principal components of “uva di Troia canosina” seed extracts by LC/ DAD analysis and the optimization of the extraction and purification processes for the development of an industrial nutraceutical product, are described. Two different fractions of seeds collected at different stages of fermentation were compared: “Tesi” 2 when there is a spontaneous stratification of the seeds at the bottom of the recipient and “Tesi” 4 at the end of fermentation. Percolation was applied and compared to maceration and the purification step carefully evaluated to obtain extracts free of contaminant species endowed with polyphenolic content comparable to commercial preparations such as Leucoselect® (Indena, SpA, Italy), Vitis Vinifera extract 95% (seeds), Vitis Vinifera dry extract 95%, Biovin grape seed and vinasse ex.. View More»
DOI:
10.4172/2157-7064.1000266