Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
+44 1223 790975
ISSN: 0974-276X
+44 1223 790975
G Wayne Zhou
Kirksville College of Osteopathic Medicine,
Kirksville, MO 63501
Tanzania
Research Article
Protein Subcellular localization profiling of Prostate Cells by Dissociable
Antibody MicroArray (DAMA) Staining Technology
Author(s): Guanyuan Fu, X Cynthia Song, Xufen Yang, Yi Mo and G Wayne ZhouGuanyuan Fu, X Cynthia Song, Xufen Yang, Yi Mo and G Wayne Zhou
Background: Dissociable Antibody MicroArray (DAMA) staining technology is a new approach for global analysis of protein expression and subcellular localization profiles in fixed cells. We have developed the technology and demonstrated its application on identifying differentially expressed proteins in breast cancer cells. We have determined the subcellular localization profiles of 360 proteins in breast cells and identified a protein with unique subcellular localization in breast cancer cells, by combining data from four 96-antibody format arrays. In this report, we have further developed the technology to determine the subcellular localization profiles of 400 proteins simultaneously from single chip, in a 400-antibody format.
Method: We have determined the subcellular localization profiles of 400 arrayed antibodies in two normal prostate ce.. View More»
DOI:
10.4172/jpb.1000382