Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
+44 1223 790975
ISSN: 0974-276X
+44 1223 790975
Frode Selheim
Jonas Lies vei 91, N-5009 Bergen
Norway
Research Article
Epac-induced Alterations in the Proteome of Human SH-SY5Y Neuroblastoma Cells
Author(s): Even Birkeland, Gyrid Nygaard, Eystein Oveland, Olav Mjaavatten, Marie Ljones, Stein Ove Doskeland, Camilla Krakstad and Frode SelheimEven Birkeland, Gyrid Nygaard, Eystein Oveland, Olav Mjaavatten, Marie Ljones, Stein Ove Doskeland, Camilla Krakstad and Frode Selheim
The hitherto proteome of the human neuroblastoma cell line SH-SY5Y consists of 1029 unique proteins. By using subcellular fractionation, SDS PAGE and nanoLC-Q-TOF-MS/MS we enlarged the proteome with additionally 72.2%, resulting in 3707 unique proteins.
The stage of neuroblastoma tumor cell differentiation is known to influence patient outcome, with a high differentiation stage correlating to favourable prognosis. To elucidate the effects of cAMP in SH-SY5Y neuroblastoma differentiation we used selective cAMP analogs to activate Epac and PKA.
We found that activation of Epac induced actin and tubulin polymerization and neurite outgrowth, whereas PKA activation did not. The effects of the Epac stimulation were abolished by knock down of Epac1 with ShRNA.
Stable isotope labelling with amino acids in cell culture (SILAC) and mass spectrometry were used to disclose.. View More»
DOI:
10.4172/jpb.1000083